Product Name: Procalcitonin Determination Kit (Fluorescence Immunoassay)
Abbreviation: PCT
APPLICATION:
For the quantitative detection of procalcitonin in human serum, plasma or whole blood.
Procalcitonin (PCT) is a hormone-active protein, mainly synthesized in the thyroid follicular cells. It is composed of three parts of N-terminal residue-calcitonin-C terminal calcitonin. It is a precursor peptide of calcitonin and an inflammatory medium with a molecular weight of 13KD. It has become a new microbiological indicator for identifying serious bacterial infections in recent years. The level of serum PCT will increase when the patient is under severe bacteria, parasites, fungal infections, sepsis or MODS. So it can be used as adjunctive diagnosis of diseases such as bacterial infections.
PRCINCIPLE:
Take the sample to the strip, the PCT in the sample is bound to the antibody coated with the fluorescent particles coated on the glass fiber to form a fluorescent particle-antibody-antigen complex. The immune complex is further chromatographed along the nitrocellulose membrane to the detection zone (T), and combined with the pre-coated mouse anti-PCT monoclonal antibody, the fluorescence intensity is proportional to the PCT content in the sample, and the remaining fluorescent antibody particle layer Factored to the QC zone (C) and bound to pre-coated goat anti-mouse IgG.
Method:
Fluorescence Immunoassay
Assay Time: 15 min
Sample Volume: 10µl
Sample Type: Serum / Plasma / Whole blood
Technical parameters:
Analytical Sensitivity: ≤0.1ng/mL
Measuring Range: 0.1 ~ 100 ng/mL
Precision: Intra-assay CV<10%, Inter-assay CV<15%.
Accuracy: Relative deviation does not exceed ±20%
Stability: Stable up to 18 months if stored at 2 - 30°C in its sealed pouch.
STORAGE AND STABILITY:
The strip is stable for 18months (while sealed in an aluminum foil pouch) if stored at 2 ° C - 30 ° C. After the strip is opened, the test should be performed in 1 hour. The date of manufacture is detailed in the label.
.
Package:
25 pieces/box
Name | Composition |
Strip | Consisting of a cartridge and a strip, the test strip comprises a sample pad, a glass fiber (coated with a fluorescent particle-PCT antibody conjugate), a nitrocellulose membrane (the detection zone is coated with a PCT monoclonal antibody, and the quality control zone is coated with a sheep) Anti-mouse IgG), absorbent paper, PVC board. |
Magcard | Have Calibration curve information of this batch reagents. |
Buffer | Main component is phosphate buffered saline (PBS). |
QC(Optional) | Freeze-dried products, the target value of each batch is shown in the target value list. |
Manual | To descripe basic information, usage methods and precautions |
Applicable POCT Analyzer
D-10, D-20, KHF01
