High Stability, Sensitivity, and Specificity Probe Synthesis
发布日期:
2023-08-11
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With the continuous development of chemical synthesis of nucleic acid technology, the use of nucleic acid probes for related research has become one of the commonly used molecular biology techniques in the field of biology and medicine. As the most commonly used signal transduction medium, nucleic acid fluorescent probes have the advantages of high analytical sensitivity, simple detection methods, and less impact on biomoleculesThey have been used to detect protein molecules, nucleic acid fragment molecules, small biological molecules, and inorganic ions, etc.


Diagreat Probe Synthesis Service

Beijing Diagreat Biotechnologies Co., Ltd has the world's leading chemical synthesis technology and automated production equipment. According to the customer's sequence or modification requirements, it can flexibly meet the needs of large-scale and high-throughput synthesis of products.


High Stability, Sensitivity, and Specificity Probe Synthesis

Using true nucleotide commercial industrial production technology, in strict compliance with the quality management system of ISO13485:2016, the whole process quality control of product production is carried out to ensure that customers are provided with stable, high-quality, g-level ultra-pure primer probe synthesis services. At present, Diagreat has provided professional services and technical support for more than 100 molecular diagnosis, genetic testing, and nucleic acid drug companies.




01

TaqMan probe fluorescent quantitative real-time PCR

TaqMan probe fluorescent quantitative real-time PCR has been widely used in various fields due to its good repeatability, high specificity  and wide linear range. The introduction of the internal standard system has further improved the sensitivity of the method and increased its reliability and practicality.


High Stability, Sensitivity, and Specificity Probe Synthesis



Diageat TaqMan Probe Synthesis Service

High Stability, Sensitivity, and Specificity Probe Synthesis

02
RPA raw material one-stop service

RPA technology uses a recombinase to combine single-stranded primers with complementary fragments in double-stranded DNA templates to initiate DNA replication without DNA melting. In recent years, experts from various countries have successively used RPA technology to detect DNA viruses and bacteria.


01

High sensitivity

02

High specificity

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Convenient operation and fast

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Quantitative analysis

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No need special instruments

Diagreat RPA exclusive probe synthesis

High Stability, Sensitivity, and Specificity Probe Synthesis

High Stability, Sensitivity, and Specificity Probe Synthesis

03

The next-generation sequencing (NGS) core material 5rApp probe

With the rapid development of high-throughput NGS technology for small RNAs, the demand for oligonucleotide joints modified by adenylation at the 5' end has greatly increased. 5'-rApp modified joints are mainly used in:


5' end tag


Activated 5'-pyrimidine-rich RNA


As an activated nucleic acid substrate for ribose/deoxyribozymes selected in vitro


Broaden the range of RNA substrates that ribose/deoxyribozymes can probe


NGS/miRNA library construction

Diagreat core product—adenosine probe

High Stability, Sensitivity, and Specificity Probe Synthesis


04

High-sensitivity LNA (locked nucleic acid) probes help RNA research

LNA Oligonucleotides can be synthesized as fully modified LNA sequences, as well as chimeras with DNA, RNA, modified bases, or tags, by conventional phosphoramidite chemistry techniques. LNA probes have extremely high affinity for nucleic acids and single-base discrimination, and can greatly improve detection sensitivity and specificity when used in Northern blotting and in situ hybridization.

Diagreat LNA Probe Synthesis Service

Customized oligonucleotide synthesis services can be provided according to the customer's design sequence or LNA modification requirements, such as modification sites, quantity, and phosphorylation requirements.

High Stability, Sensitivity, and Specificity Probe Synthesis

DIA-UP BIOTECH
【Diagreat Products】


High Stability, Sensitivity, and Specificity Probe Synthesis

DIA-UP BIOTECH

Performance Analysis of Diagreat Probe


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CY5

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Layout diagram




High Stability, Sensitivity, and Specificity Probe Synthesis




 

Ct value




High Stability, Sensitivity, and Specificity Probe Synthesis

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Amplification curve




High Stability, Sensitivity, and Specificity Probe Synthesis

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Puc19

Comparison of sensitivity and signal value with well-known foreign brands:


High Stability, Sensitivity, and Specificity Probe Synthesis

High Stability, Sensitivity, and Specificity Probe Synthesis




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