CN
|
EN
With the continuous development of chemical synthesis of nucleic acid technology, the use of nucleic acid probes for related research has become one of the commonly used molecular biology techniques in the field of biology and medicine. As the most commonly used signal transduction medium, nucleic acid fluorescent probes have the advantages of high analytical sensitivity, simple detection methods, and less impact on biomolecules. They have been used to detect protein molecules, nucleic acid fragment molecules, small biological molecules, and inorganic ions, etc.
Beijing Diagreat Biotechnologies Co., Ltd has the world's leading chemical synthesis technology and automated production equipment. According to the customer's sequence or modification requirements, it can flexibly meet the needs of large-scale and high-throughput synthesis of products.
Using true nucleotide commercial industrial production technology, in strict compliance with the quality management system of ISO13485:2016, the whole process quality control of product production is carried out to ensure that customers are provided with stable, high-quality, g-level ultra-pure primer probe synthesis services. At present, Diagreat has provided professional services and technical support for more than 100 molecular diagnosis, genetic testing, and nucleic acid drug companies.
TaqMan probe fluorescent quantitative real-time PCR
TaqMan probe fluorescent quantitative real-time PCR has been widely used in various fields due to its good repeatability, high specificity and wide linear range. The introduction of the internal standard system has further improved the sensitivity of the method and increased its reliability and practicality.
RPA technology uses a recombinase to combine single-stranded primers with complementary fragments in double-stranded DNA templates to initiate DNA replication without DNA melting. In recent years, experts from various countries have successively used RPA technology to detect DNA viruses and bacteria.
High sensitivity
High specificity
Convenient operation and fast
Quantitative analysis
No need special instruments
The next-generation sequencing (NGS) core material 5rApp probe
With the rapid development of high-throughput NGS technology for small RNAs, the demand for oligonucleotide joints modified by adenylation at the 5' end has greatly increased. 5'-rApp modified joints are mainly used in:
◇5' end tag
◇Activated 5'-pyrimidine-rich RNA
◇As an activated nucleic acid substrate for ribose/deoxyribozymes selected in vitro
◇Broaden the range of RNA substrates that ribose/deoxyribozymes can probe
◇NGS/miRNA library construction
High-sensitivity LNA (locked nucleic acid) probes help RNA research
LNA Oligonucleotides can be synthesized as fully modified LNA sequences, as well as chimeras with DNA, RNA, modified bases, or tags, by conventional phosphoramidite chemistry techniques. LNA probes have extremely high affinity for nucleic acids and single-base discrimination, and can greatly improve detection sensitivity and specificity when used in Northern blotting and in situ hybridization.
Customized oligonucleotide synthesis services can be provided according to the customer's design sequence or LNA modification requirements, such as modification sites, quantity, and phosphorylation requirements.
【Performance Analysis of Diagreat Probe】
Layout diagram
Ct value
Amplification curve
Comparison of sensitivity and signal value with well-known foreign brands:
Related Recommendations
Chat with us
E-mail inquiries