Troponin Tn

Troponin

When cardiomyocytes are injured and necrotic, cTn (cardiac troponin) is released into the peripheral blood through the damaged cell membrane. Cardiac troponin is a complex formed by three subunits of troponin C, troponin I and troponin T. In the blood of AMI patients, cTnT mainly exists in free form, while cTnI and cTnC exist in the form of complexes. The detection level of early cTnI and cTnT detection systems is ng/ml, while the modern high-sensitivity detection system can reach the level of pg/ml, and potential AMI patients can be found in the first 1-3 hours. This development has improved the level of diagnosis and risk stratification of ACS, and also made troponin (Tn) a recognized indicator with high sensitivity and high specificity in clinical practice and is recommended by all guidelines.

Break down technical barriers

Differentiated innovation of DIA-UP special protein troponin series products

As the world's leading upstream platform company for in vitro diagnostics, DIA-UP has been deeply involved in the core biological raw materials of reagents for more than ten years and is a professional supplier of special protein and small molecule raw materials. Through repeated tests and a large number of experimental verifications by the core R&D team, it is found that most of the cardiac troponin T recombinant antigens currently on the market are obtained through inclusion body renaturation. Therefore, it often contains denaturants such as urea, and the protein structure is different from the endogenous cTnT in clinical samples. In response to this difficult problem, DIA-UP has innovatively developed a soluble recombinant cTnT protein, and the entire production process does not contain any denaturants.

01 Recommended Troponin Key Raw Materials

DIA-UP BIOTECH Troponin (cTn)

02 Core performance verification

DIA-UP BIOTECH cTnI recombinant antigen

a soluble expression

No purification label, no protein denaturant (SDS, urea, guanidine hydrochloride).

b high stability

The preparation of 20-200 pg/mL calibrator has good stability (acceleration of 7 days at 37 degrees <10%).

c Wide applicability

Fusion expression of cTnI and cTnC, suitable for reagents targeting cTnI and cTnI/C complex.

DIA-UP BIOTECH cTnT recombinant antigen

a soluble expression

No purification label, no protein denaturant (SDS, urea, guanidine hydrochloride).

b high activity

The cTnT reagent assay has good dilution linearity and activity.

c High stability

The preparation of 20-200 pg/mL calibrator has good stability (acceleration of 7 days at 37 degrees <10%).

cTnT recombinant antigen

Use self-made hs-cTnT chemiluminescence reagent to test cTnT recombinant antigen at different dilutions.

DIA-UP BIOTECH

Natural cTn complex antigen

a Wide applicability

It is suitable for the development of calibrator and quality control products of cTnI and cTnT reagents.

b high activity

Naturally extracted, the conformational epitope is better maintained.

DIA-UP BIOTECH cTnI/cTnT Stripped Matrix

DIA-UP uses affinity chromatography to remove the cTnT/cTnI protein in the sample to obtain a natural negative matrix, which is especially suitable for the preparation of cTnT calibrator and quality control product, and the matrix is basically the same as the real sample, which can effectively improve the reliability of the detection of high-sensitivity cTn items among different devices.

To inquire about special protein troponin series products, please contact us through the following ways——

Tel : 185 1363 7192

E-mile : order@dia-up.com

Building 18, No.2 Yard, Huanke Middle Road, Tongzhou District, Beijing