One-stop service for RPA raw materials | Ultra-high-purity RPA probes simplify the research and development of nucleic acid isothermal amplification!
Recombinase polymerase amplification (RPA) is known as the only nucleic acid isothermal amplification method that can replace PCR technology. Compared with PCR technology, RPA has outstanding advantages such as shorter time-consuming, lower cost, more accurate amplification of target genes, and lower equipment dependence. It has higher application prospects and has been widely used in the field of agriculture, food, medicine, biological point-of-care detection.
| Recombinase cofactor etc. | Recombinase cofactor etc. |
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Form Recombinase-primer complex, and the complex searched for homologous sequences of target genes. |
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| Repeated, exponential expansion |
The strands exchange to form a D-ring structure. |
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DNA polymerase recognizes free 3’-OH of primers and initiates the amplification reaction. |
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The parental strand is separated, and the DNA daughter strand is synthesized. |
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Two DNA daughter strands are formed. |
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Recombinase | Single-strand binding protein | Forward/reverse primer | DNA polymerase |
One of the important factors affecting RPA amplification - probe
Most of the probes used in the RPA reaction are long probes, and short probes will affect the recombination efficiency of the RPA reaction. In the reaction system where probes need to be added, the probe concentration is also a key factor affecting the experimental results, usually the probe concentration is 120 nmol/L. In addition, different primers and probe combinations have different amplification efficiencies, and multiple screenings are required to achieve experimental expectations. Probe base mismatches will affect the amplification efficiency and cause false positive results.
Probe method developed based on RPA
DIAGREAT RPA exclusive probe synthesis service
The probe is one of DIA-UP's characteristic service products, with a 100,000-class clean room, a high-throughput automation platform, and pharmaceutical-grade quality control standards. Through technical research and lean production, the consistency and stability of products are enhanced, and a wide variety of modified primers are provided. At present, it has provided professional services and high-quality core raw materials for more than 100 molecular diagnosis, genetic testing, and nucleic acid drug companies around the world.
Core advantages
‖ High sensitivity
‖ High specificity
‖ Positive results in as little as 8 minutes
‖ HPLC detection purity ≥ 95% and customizable higher purity standards
‖Strictly implement the quality management system ISO13485:2016, GMP standard management
Performance display
Mass Spectrum - Molecular Weight: 17124.4
UPLC detection chart - purity 95%
Auto-scale chromatogram
minute
Peak results
Name
Retention time (min)
Area (μV*s)
Hight (μV)
% area
RPA key raw material one-stop service
