Total prostate specific antigen(tPSA) CLIA kit (Chemiluminescence Immunoassay) is an in vitro diagnostic quantitative test kit. Screening approaches have reduced prostate cancer mortality in the USA and in Europe. The most widely used screening tests for prostate cancer are digital rectal examination (DRE) in combination with serum total(free + complexed) PSA.Total PSA can also be used to do active surveillance, assess therapy response and for post-treatment follow up.