5'nucleotidase(5NT) Determination Reagent is an in vitro diagnostic quantitative test kit. 5′ nucleotidase is used to investigate the origin of increased serum alkaline phosphatase. It is a liver-related enzyme used to work up cholestatic/biliary obstruction. It is increased with metastatic neoplasia in liver and in primary biliary cirrhosis, biliary obstruction from calculi and tumor