RNA Intelligent And Synthesis Platform

Diagreat RNA intelligent design and synthesis platform has the core technology of RNA chemical synthesis, which reaches the international leading level. We have a variety of services, including general siRNA synthesis, siRNA modification and labelling, siRNA design, miRNA design and synthesis, and common siRNA control which can meet the different scientific research needs of high quality customers.


—— siRNA ——

Introduction

Small interfering RNA(siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA molecules, 20-25 nucleotides in length, that play a variety of roles in biology. They are similar to miRNA, and works within the RNA interference(RNAi). siRNA interferes with the expression of specific genes with complementary nucleotide sequences by affecting the degradation of mRNA post-transcription, thus preventing translation. siRNA synthesis is the simplest and fastest method to achieve gene silencing in organism and cell. It has the advantages of high transfection efficiency with little toxicity, and large scale preparation. It is especially suitable for screening effective fragments of siRNA in the case of uncertain gene target sites. siRNA has irreplaceable advantages in gene function studies , drug target and drug screening.


【Advantages】

1.Professional project management team, comprehensive professional pre-sales consulting, order design and after-sales service.

2.ISO13485 strict quality control standards, GMP factory, and comprehensive quality control report( HPLC/MS).

3.The synthetic specifications are flexible and diverse, and can be customized in batches.

4.Provide personalized customized service, such as special service and preferential package services, etc.


【Special Services】

1.siRNA Design and Synthesis

Based on the target gene name, sequence or GeneBank ID, etc, Dia-up will design siRNA free of charge to ensure that at least one pair can effectively inhibit the expression of target genes. It will be redesigned and synthesized for free, if the silencing effect is not achieved.

2.Modified or labeled siRNA synthesis

Various modified and labeled siRNA synthesis can be carried out according to customer requirements. Custom modified or labeled siRNA synthesis is also available.

3.siRNA screening

Dia-up provides effective siRNA screening services, helps customers screening out the most effective siRNA, and provides detailed experimental reports, including experimental methods, procedures, photos and relevant data.


【Single-gene Package】

Package A: Genral siRNA economical package

ProductsQuantitySpecificationPurification
Target gene siRNA oligos3 pairs3*2ODHPLC
Negative control siRNA oligos1 pairs1*1ODHPLC
FAM labeled negative control1 pairs1*1ODHPLC
Positive control siRNA oligos1 pairs1*1ODHPLC


Package B: Genral siRNA economical package

ProductsQuantitySpecificationPurification
Target gene siRNA oligos4 pairs4*4ODHPLC
Negative control siRNA oligos1 pairs1*1ODHPLC
FAM labeled negative control1 pairs1*1ODHPLC
Positive control siRNA oligos1 pairs1*1ODHPLC


Package C: Chemical modified siRNA economical package

ProductsQuantitySpecificationPurification
Target gene siRNA oligos3pairs3*2ODHPLC
Negative control siRNA oligos1 pairs1*1ODHPLC
FAM labeled negative control1 pairs1*1ODHPLC
Positive control siRNA oligos1 pairs1*1ODHPLC


Package D: Chemical modified siRNA affordable package

ProductsQuantitySpecificationPurification
Target gene siRNA oligos4pairs4*4ODHPLC
Negative control siRNA oligos1 pairs1*1ODHPLC
FAM labeled negative control1 pairs1*1ODHPLC
Positive control siRNA oligos1 pairs1*1ODHPLC


Package E: Fluorescent dye labeled siRNA economical package

ProductsQuantitySpecificationPurification
Target gene siRNA oligos3pairs3*2ODHPLC
Negative control siRNA oligos1 pairs1*1ODHPLC
FAM labeled negative control1 pairs1*1ODHPLC
Positive control siRNA oligos1 pairs1*1ODHPLC


Package F: Fluorescent dye labeled siRNA affordable package

ProductsQuantitySpecificationPurification
Target gene siRNA oligos4pairs4*4ODHPLC
Negative control siRNA oligos1 pairs1*1ODHPLC
FAM labeled negative control1 pairs1*1ODHPLC
Positive control siRNA oligos1 pairs1*1ODHPLC


【Service Procedure】

RNA Intelligent And Synthesis  Platform


【Project Delivery Result】

  • Lyophilized Powder of Synthesis RNA 

  • COA document 

  • Test report (MS & HPLC)


【Order Information】

RNA Synthesis


Product typeSpecificationProduct nameDelivery
General siRNA2 ODGeneral siRNA synthesis5 working days
3 OD5 working days
4 OD5 working days
5 OD5 working days
6 OD5 working days
7 OD5 working days
8 OD5 working days
9 OD5 working days
10 OD5 working days
25 OD5 working days
50 OD5 working days
100 OD5 working days
Above 250 ODUpon request
General siRNA package A3pairs(3X2 0D)

1pairs(1X1 0D)

1pairs(1X1 0D)

1pairs(1X1 0D)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligo

5 working days
General siRNA package B

4pairs(4X4 0D)

1pairs(1X1 0D)

1pairs(1X1 0D)

1pairs(1X1 0D)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligo

5 working days


Product typeSpecificationProduct nameDelivery
Chemical modified siRNA package C (thio, oligo, amino, sulfhydryl)

3 pairs (3X2 OD

1 pair (1X1 OD) 

1 pair (1X1 OD) 

1 pair (1X1 OD) 

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligos


5 working days
Chemical modified siRNA package D (thio, oligo, amino, sulfhydryl)

4 pairs (4X4 OD

1 pair (1X1 OD)

1 pair (1X1 OD) 

1 pair (1X1 OD)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligos

5 working days
Fluorescent dye labeled siRNA package E (FAM, HEX, Cy3)

3 pairs (3X2 OD)

1 pair (1X1 OD) 

1 pair (1X1 OD) 

1 pair (1X1 OD)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligos

5 working days
Fluorescent dye labeled siRNA package F (FAM, HEX, Cy3)

4 pairs (4X4 OD)

1 pair (1X1 OD) 

1 pair (1X1 OD) 

1 pair (1X1 OD)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligos

5 working days
In vivo siRNA package G (Full chain methoxy modification, terminal cholesterol modification)
3 pairs (3X2 OD) 

1 pair (1X1 OD) 

1 pair (1X1 OD) 

1 pair (1X1 OD)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligos

8 working days
In vivo siRNA package H (Full chain methoxy modification, terminal cholesterol modification)

3  pairs (4X4 OD)

1 pair (1X1 OD) 

1 pair (1X1 OD) 

1 pair (1X1 OD)

Target gene siRNA oligos

Negative control siRNA oligos

FAM labeled negative control siRNA oligos

Positive control siRNA oligos

8 working days
Fluorescent dye labeled siRNA

4  OD

5  OD

10 OD

25 OD

50 OD

100 OD

250 OD
Fluorescent dye labeled siRNA

7 working days

7 working days 

7 working days 

11 working days 

7 working days 

16 working days 

21 working days


RNA Modification Type

TypeModification markerPurificationTypeModificationPurification
Rare basedI

rI

m5rC

m6rA

2'-0-MethylRNA

2'F RNA

m7rG

LNA-X

mlrA

m5Cm

m5Um


HPLCOther modification

Spacer9

Azide

DBCO

Spacer(C6)

Spacer9

Spacer(C12)

Spacer C3

Spacer C18

dSpacer

PL

Int Amino dT

Int Biotin dT

Int Cy3 dT

Int Cy5 dT

Int TAMRA dT

Int ROX dT

Int FAM dT

Int HEX dT

Int Dig dT

Int DBCO dT

Int TAMRA dT

Int ATTO Flour dT

Int BHQ1 dT

Int BHQ2 dT

Int NPOM Dt

HPLC
Thiomodification (Phosphorothioates)SHPLCNH2 modification (Amino Linkers)

5'Amino C6

3' Amino C7

HPLC
Phosphorylation

5'PHO 

 3' PHO

HPLCSH modification (Thiol)

3'Thiol C3

5'Thiol C6

HPLC
Biotin

5'Biotin

3'Biotin TEG

5'Desthiobiotin

3'Desthiobiotin

HPLC(Digoxigenin)

5'DI

G3' DIG

HPLC
(Fluorescence)

5' Cy3

3' Cy3

5’Cy5

3’Cy5

5’FAM

3’FAM

5’HEX

3’HEX

5’-FITC

3’-FITC

5’6-JOE

3’6-JOE

5’ROX

3’ROX

5’TAMRA

3’TAMRA

5’Texas Red

3’Texas Red


HPLC(Quenchers)

3’DABCYL

3’TAMRA3' BHQ 1

3’BHQ 2

3’BHQ3

3’-MGB

3’TQ1-TQ7系列

3'eclipse

HPLC
(Fluorescence)

5'Quasar 570

3'Quasar 570

5'Quasar 670

3'Quasar 670

5'Cy5. 5NS

3' Cy5. 5NS

5'Cy7NS

3' Cy7NS

5'&3DABCYL

5'83'AMCA

5'-VIC

HPLC


Double Labeled Modification

TypeModification markerPurificationTypeModificationPurification
TAMRA

5’FAM-3’TAMRA

5’HEX-3’TAMRA

5’TET-3’TAMRA

5’JOE-3’TAMRA

5'  VIC-3'  TAMRA

HPLCBHQ1

5’FAM-3’BHQ1

5’VIC-3’BHQ1

5’HEX-3’BHQ1

HPLC
ECLIPS

5’FAM-3’ECLIPS

5’HEX-3’ECLIPS

5' TAMRA-3’Eclipse

5' ROX-3’Eclipse

5' TET-3’Eclipse

5' JOE-3’Eclipse5

HPLCMGB

5’FAM-3' MGB

5’HEX-3' MGB

5’ROX-3' MGB

5’Texas Red-3’MGB

5’Quasar 670-3’MGB

5’ VIC-3’MGB

HPLC
BHQ2

5' Cy5-3' BHQ2

5' Cy3-3' BHQ2

5' ROX-3' BHQ2

5' ROX-3' BHQ2

5' TET-3' BHQ2

5' JOE-3' BHQ2

5' HEX-3' BHQ2

5' TAMRA-3' BHQ2

5' Texas Red-3' BHQ2

5' Quasar 670-3' BHQ2

5' -FAM and 3' BHQ2

HPLCMGB

5' FAM-3'MGB

5' HEX-3'MGB

5'ROX-3'MGB

5'Texas Red-3'MGB

5'Quasar 670-3'MGB

5' VIC-3'MGB

HPLC
Dabcyl

5' FAM-3' DABCYL

5' HEX-3' DABCYL

5' TAMRA-3' DABCYL

5'Texas Red-3'Dabcyl

5'ROX-3'Dabcyl

5'CY3-3'DABCYL

5' Cy5-3' DABCYL

HPLC
Other double labeled modification

5' Cy5,3' SH

5' Dabcyl,3' C3-Fam

5' DlG,3' Digoxin

5' SH,3' Cy3

5' Fam,3' C3-Fam

5' TAMRA 3' C3-Fam

5' Cy5,3' C7-NH2

HPLCOther double labeled modification

5'Phos and 3'FAM

5'TAMRA and 3' Phos

5' C6-Biotin,3' Biotin

5' C6-Biotin,3' Cy5

5' C6-NH2,3' C3-Fam

5' C6-NH2,3' Cy3

5' Cy5,SS,

HPLC
BHQ3

5' Quasar 670-3' BHQ3

5' CY5-3' BHQ3

HPLC


* Other modification types upon request.


 —— miRNA —— 


MicroRNAs (miRNAs) are endogenous single-stranded RNAs of 18-25nt in eukaryotic organisms, which can regulate the complementary mRNAs at the post-transcriptional level through cleavage or translational repression of the mRNA targets.According to the recent studies, miRNA are involved in a variety of regulatory pathways, including development, viral defense, hematopoietic processes, organogenesis, cell proliferation, apoptosis, and fat metabolism. Dia-up’s advanced synthesis technology can provide customers with miRNA inhibitors, miRNA mimics, miRNA negative controls, Agomir and Antagomir.


【Unique Products】


miRNA inhibitors

Introduction

  • miRNA inhibitors are the chemically modified inhibitors which specifically target miRNA in cells.

Advantages

  • ffectively inhibit endogenous mature miRNA function 

  • low concentrations of miRNA inhibitor can effectively inhibit miRNA activity for a long time

Application

  • the Loss-of-function effect can be further investigated with microRNA, which can reduce the miRNA activity by GMR-miRTM microRNA inhibitors inhibition

  • Screening for miRNAs that regulate the expression of a gene

  • Further study the function of miRNA in biological process, such as cell development, proliferation, differentiation and apoptosis.


miRNA mimics

Introduction

  • miRNA mimics are chemically synthesized and optimized nucleic acids designed to mimic endogenous mature miRNAs molecules in cells, and can enhance the function of endogenous mature miRNAs.

Advantages

  • convenient and efficient, can be transfected into cells and play a role.

  • The operation is simple and can be transfected into cells with transfection reagent withoutconstructing the vector, and the transfection efficiency can be studied t hrough transfection control.

  • Short experiment period 

  • Has a wide range of application


Agomir and Antagomir

Introduction

  • Agomir is specially labeled and chemically modified miRNA agonist. Agomir can regulate the biological function of the target gene by mimicking endogenous microRNAs.

  • Antagomir, with a design that is based on the mature microRNA sequence, is chemically modified miRNA antagonist.

Advantages

  • Compared with common miRNA inhibitors and mimics, both Antagomir and Agomir have a higher affinity for the cell membrane. Therefore, the amount of transfection reagent could be drastically reduced.

  • Has a wide range of application, especially suitable for animals and cell experiment.

  • Easy to operate, and can be injected into the animal by method of intravenous or local injection.

  • The interfering effect can last a long time, at least one week. The longest interfering effect can be up to 5-6 weeks.

  • They can be enriched in target cells, inducing effective, specific and stable interfering effects.

Application

Specific microRNA Agomir can be introduced into cells which express the corresponding microRNA, and then it simulates the effect of microRNA to simulate/inhibit the effect of microRNA. Or the specific microRNA Agomir/Antagomir can be used to combine with the double luciferase reporter system/suppress the expression of a reporter vector, to verify the regulatory relationship between the miRNA and the target gene.


【Advantages】

  • Professional project management team, comprehensive professional pre-sales consulting, order design and after-sales service.

  • ISO13485 strict quality control standards, GMP factory, and comprehensive quality control report( HPLC/MS).

  • The synthetic specifications are flexible and diverse, and can be customized in batches.

  • Provide personalized customized service, such as special service and preferential package services, etc.



【Service Procedure】

RNA Intelligent And Synthesis  Platform


【Project Delivery Result】

  • Lyophilized Powder of Synthesis RNA 

  • COA document 

  • Test report (MS & HPLC)


【Order Information】

Product typeSpecificationProduct nameDelivery
microRNA2OD

microRNA mimics

microRNA inhibitor

microRNA mimics N.C

microRNA inhibitor N.C

microRNA agomiR

microRNA antagomiR

microRNA agomiR N.C

microRNA antagomiR N.C

5 working days



【Unique Products】

Dia-up Biotech provides common RNA control for the convenience of all scientific customers. All product ready to ship.


Product typeSpecificationProduct name
Control siRNA1 OD

Negative control siRNA

FAM labeled negative control siRNA

GAPDH positive control siRNA

P53 positive control siRNA

β-actin positive control siRNA

Mimics N.C

Inhibitor N.C


 —— mRNA —— 


mRNA synthesis for RNA vaccine and medicine R&D