Diagreat RNA intelligent design and synthesis platform has the core technology of RNA chemical synthesis, which reaches the international leading level. We have a variety of services, including general siRNA synthesis, siRNA modification and labelling, siRNA design, miRNA design and synthesis, and common siRNA control which can meet the different scientific research needs of high quality customers.

—— siRNA ——

【Introduction】

Small interfering RNA（siRNA）, sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA molecules, 20-25 nucleotides in length, that play a variety of roles in biology. They are similar to miRNA, and works within the RNA interference（RNAi）. siRNA interferes with the expression of specific genes with complementary nucleotide sequences by affecting the degradation of mRNA post-transcription, thus preventing translation. siRNA synthesis is the simplest and fastest method to achieve gene silencing in organism and cell. It has the advantages of high transfection efficiency with little toxicity, and large scale preparation. It is especially suitable for screening effective fragments of siRNA in the case of uncertain gene target sites. siRNA has irreplaceable advantages in gene function studies , drug target and drug screening.

【Advantages】

1.Professional project management team, comprehensive professional pre-sales consulting, order design and after-sales service.

2.ISO13485 strict quality control standards, GMP factory, and comprehensive quality control report（ HPLC/MS）.

3.The synthetic specifications are flexible and diverse, and can be customized in batches.

4.Provide personalized customized service, such as special service and preferential package services, etc.

【Special Services】

1.siRNA Design and Synthesis

Based on the target gene name, sequence or GeneBank ID, etc, Dia-up will design siRNA free of charge to ensure that at least one pair can effectively inhibit the expression of target genes. It will be redesigned and synthesized for free, if the silencing effect is not achieved.

2.Modified or labeled siRNA synthesis

Various modified and labeled siRNA synthesis can be carried out according to customer requirements. Custom modified or labeled siRNA synthesis is also available.

3.siRNA screening

Dia-up provides effective siRNA screening services, helps customers screening out the most effective siRNA, and provides detailed experimental reports, including experimental methods, procedures, photos and relevant data.

【Single-gene Package】

Package A: Genral siRNA economical package

Package B: Genral siRNA economical package

Package C: Chemical modified siRNA economical package

Package D: Chemical modified siRNA affordable package

Package E: Fluorescent dye labeled siRNA economical package

Package F: Fluorescent dye labeled siRNA affordable package

【Service Procedure】

【Project Delivery Result】

• Lyophilized Powder of Synthesis RNA 

• COA document 

• Test report (MS & HPLC)

【Order Information】

RNA Synthesis

RNA Modification Type

Double Labeled Modification

* Other modification types upon request.

 —— miRNA —— 

MicroRNAs (miRNAs) are endogenous single-stranded RNAs of 18-25nt in eukaryotic organisms, which can regulate the complementary mRNAs at the post-transcriptional level through cleavage or translational repression of the mRNA targets．According to the recent studies, miRNA are involved in a variety of regulatory pathways, including development, viral defense, hematopoietic processes, organogenesis, cell proliferation, apoptosis, and fat metabolism. Dia-up’s advanced synthesis technology can provide customers with miRNA inhibitors, miRNA mimics, miRNA negative controls, Agomir and Antagomir.

【Unique Products】

miRNA inhibitors

Introduction

• miRNA inhibitors are the chemically modified inhibitors which specifically target miRNA in cells.

Advantages

• ffectively inhibit endogenous mature miRNA function 

• low concentrations of miRNA inhibitor can effectively inhibit miRNA activity for a long time

Application

• the Loss-of-function effect can be further investigated with microRNA, which can reduce the miRNA activity by GMR-miRTM microRNA inhibitors inhibition

• Screening for miRNAs that regulate the expression of a gene

• Further study the function of miRNA in biological process, such as cell development, proliferation, differentiation and apoptosis.

miRNA mimics

Introduction

• miRNA mimics are chemically synthesized and optimized nucleic acids designed to mimic endogenous mature miRNAs molecules in cells, and can enhance the function of endogenous mature miRNAs.

Advantages

• convenient and efficient, can be transfected into cells and play a role.

• The operation is simple and can be transfected into cells with transfection reagent withoutconstructing the vector, and the transfection efficiency can be studied t hrough transfection control.

• Short experiment period 

• Has a wide range of application

Agomir and Antagomir

Introduction

• Agomir is specially labeled and chemically modified miRNA agonist. Agomir can regulate the biological function of the target gene by mimicking endogenous microRNAs.

• Antagomir, with a design that is based on the mature microRNA sequence, is chemically modified miRNA antagonist.

Advantages

• Compared with common miRNA inhibitors and mimics, both Antagomir and Agomir have a higher affinity for the cell membrane. Therefore, the amount of transfection reagent could be drastically reduced.

• Has a wide range of application, especially suitable for animals and cell experiment.

• Easy to operate, and can be injected into the animal by method of intravenous or local injection.

• The interfering effect can last a long time, at least one week. The longest interfering effect can be up to 5-6 weeks.

• They can be enriched in target cells, inducing effective, specific and stable interfering effects.

Application

Specific microRNA Agomir can be introduced into cells which express the corresponding microRNA, and then it simulates the effect of microRNA to simulate/inhibit the effect of microRNA. Or the specific microRNA Agomir/Antagomir can be used to combine with the double luciferase reporter system/suppress the expression of a reporter vector, to verify the regulatory relationship between the miRNA and the target gene.

【Advantages】

• Professional project management team, comprehensive professional pre-sales consulting, order design and after-sales service.

• ISO13485 strict quality control standards, GMP factory, and comprehensive quality control report（ HPLC/MS）.

• The synthetic specifications are flexible and diverse, and can be customized in batches.

• Provide personalized customized service, such as special service and preferential package services, etc.

  
  

【Service Procedure】

【Project Delivery Result】

• Lyophilized Powder of Synthesis RNA 

• COA document 

• Test report (MS & HPLC)

【Order Information】

【Unique Products】

Dia-up Biotech provides common RNA control for the convenience of all scientific customers. All product ready to ship.

 —— mRNA —— 

mRNA synthesis for RNA vaccine and medicine R&D