DNA Primer and Probe Synthesis Service

【Introduction】

GMP probes and primers are the featured products of Beijing Diagreat Biotechnologies Co.,Ltd .They are produced under GMP, and can be applied to clinical application and molecular diagnostics. GMP helps this production changes from research to commercialization. Clients can customize the synthesis and the purification type of the probes and primers.DNA Primer and Probe Synthesis Service

【Features】

1.GMP Factory

2.A wide variety of probes modifications and specifications

3.Provide high affinity and ultra-high affinity purification primers and probes

4.Provide commercial detection kit and IVD reagents.


【IVD qPCR Probes Modified Bases】

Common Fluorophores

DescriptionDescription3’ modificationsIntermediate modificationsMaximum Excitation SpectraMaximum Emission SpectraSpectra Color
AMCA
350 nm440 nm
Pacific Blue√dT410 nm455 nm
Atto 425√dT436 nm484 nm
BODIPY FL√dT503 nm512 nm
FAM√dT494 nm518 nm
Alexa Fluor 488√dT496 nm519 nm
TET√dT521 nm536 nm
JOE√dT520 nm548 nm
Yakima Yellow

530 nm549 nm
VIC

538 nm554nm
HEX√dT535 nm556 nm
Quasar 570√dT √skeleton547 nm570 nm
Cy3√dT √skeleton552 nm570 nm
NED

546 nm575 nm
TAMRA√dT565 nm580 nm
ROX√dT585 nm605 nm
AquaPhluor 593

593 nm613 nm
Texas red√dT595 nm615 nm
Atto 590
594 nm624 nm
Cy5√dT √skeleton643 nm667 nm
Quasar 670
647 nm667 nm
Cy5.5√dT684 nm710 nm


Common Quenchers


Description5’ modifications3’ modificationsIntermediate modificationsThe range of quenchers spectra
Dabcyl√dT380 nm-530 nm453 nm
Eclipse

390 nm-625 nm522 nm
MGB

390 nm-625 nm522 nm
MGB√dT480 nm-580 nm534 nm
BHQ2√dT550 nm-650 nm579 nm
BHQ3

620 nm-730 nm672 nm


Common Fluorophores and Quenchers Pairs

DescriptionTaqman QuenchersMGB QuenchersMolecular Beacon Quenchers
BHQ1BHQ2BHQ3MGBDabcyl
Pacific Blue




Atto 425

BODIPY FL

FAM

Alexa Fluor 488

TET

JOE

Yakima Yellow

VIC

HEX

Quasar 570



Cy3



NED



TAMRA



ROX



AquaPhluor 593



Texas red



Atto 590



Cy5



Quasar 670



Cy5.5





【Product List】



General primerClassificationDNA Length
Conventional Primer Synthesis11-59mer
ExplainationAll products are purified by HPLC


Product TypeModificationsProduct TypeModifications
Single-labeled ProbesLNA(bp)Dual-labeled Probes5′ FAM 3′ TAMRA
DI(bp)5′ FAM 3′ BHQ1
DU(bp)5′ FAM 3′ Dabcy1
sufo(bp)5′ FAM 3′ MGB
Spacer C3(bp)5′ FAM 3′ Eclipse
Spacer 9(bp)5′ HEX 3′ BHQ1
Spacer 12(bp)5′ HEX 3′ TAMRA
Spacer 18(bp)5′ HEX 3′ Eclipse
dSpacer 5′ HEX 3′ Dabcy1
dT-C6-Amino5′ HEX 3′ MGB
5′ -SH5′ ROX 3′ BHQ2
5′ NH2 C65′ ROX 3′ MGB
NH2 C125′ Cy5 3′ BHQ2
3′  NH2 C75′ Cy5 3′ BHQ3
5′ -Phosphorylation5′ Cy5 3′ MGB
3′ -Phosphorylation5′ Cy5.5 3′ BHQ3
5′ Biotin5′ TET 3′ BHQ1
3′ Biotin5′ TET 3′ TAMRA
5′ FAM5′ VIC 3′ Dabcy1
3′ FAM5′ VIC 3′ BHQ1
5′ HEX5′ VIC 3′ TAMRA
5′ TET5′ VIC 3′ MGB
5′ TAMRA(dT) probesdT-FAM
3′ TAMRAdT-ROX
5′ ROXdT-TAMRA
3′ ROXdT-Dabcy1
5′ Cy3dT-Cy3
3′ Cy3dT-Cy5
5′ Cy5Single-labeled Probes3′ -Dabcy1
Cy5.55′ -Dabcy1
3′ Cy53′ BHQ1
5′ VIC3′ BHQ2

3′ddA/ddT/ddC/ddG

3′ BHQ3
5′rapp3′ eclipse

3′ -MGB

*Other modification types are upon requested.