【Introduction】
GMP probes and primers are the featured products of Beijing Diagreat Biotechnologies Co.,Ltd .They are
produced under GMP, and can be applied to clinical application and molecular diagnostics. GMP helps this
production changes from research to commercialization. Clients can customize the synthesis and the
purification type of the probes and primers.
【Features】
1.GMP Factory
2.A wide variety of probes modifications and
specifications
3.Provide high affinity and ultra-high
affinity purification primers and probes
4.Provide commercial detection kit and IVD
reagents.
【IVD qPCR Probes Modified Bases】
Common Fluorophores
| Description | Description | 3’ modifications | Intermediate
modifications | Maximum
Excitation Spectra | Maximum
Emission Spectra | Spectra Color |
| AMCA | √ | √ |
| 350 nm | 440 nm |
|
| Pacific Blue | √ | √ | √dT | 410 nm | 455 nm |
| Atto 425 | √ | √ | √dT | 436 nm | 484 nm |
| BODIPY FL | √ | √ | √dT | 503 nm | 512 nm |
|
| FAM | √ | √ | √dT | 494 nm | 518 nm |
| Alexa Fluor 488 | √ | √ | √dT | 496 nm | 519 nm |
| TET | √ | √ | √dT | 521 nm | 536 nm |
|
| JOE | √ | √ | √dT | 520 nm | 548 nm |
| Yakima Yellow |
| √ |
| 530 nm | 549 nm |
| VIC | √ |
|
| 538 nm | 554nm |
|
| HEX | √ | √ | √dT | 535 nm | 556 nm |
| Quasar 570 | √ | √ | √dT √skeleton | 547 nm | 570 nm |
|
| Cy3 | √ | √ | √dT √skeleton | 552 nm | 570 nm |
| NED | √ |
|
| 546 nm | 575 nm |
| TAMRA | √ | √ | √dT | 565 nm | 580 nm |
| ROX | √ | √ | √dT | 585 nm | 605 nm |
|
| AquaPhluor 593 |
| √ |
| 593 nm | 613 nm |
| Texas red | √ | √ | √dT | 595 nm | 615 nm |
| Atto 590 | √ | √ |
| 594 nm | 624 nm |
| Cy5 | √ | √ | √dT √skeleton | 643 nm | 667 nm |
|
| Quasar 670 | √ | √ |
| 647 nm | 667 nm |
| Cy5.5 | √ | √ | √dT | 684 nm | 710 nm |
Common Quenchers
| Description | 5’ modifications | 3’ modifications | Intermediate
modifications | The range of quenchers spectra |
|
| Dabcyl | √ | √ | √dT | 380 nm-530 nm | 453 nm |
| Eclipse |
| √ |
| 390 nm-625 nm | 522 nm |
| MGB |
| √ |
| 390 nm-625 nm | 522 nm |
| MGB | √ | √ | √dT | 480 nm-580 nm | 534 nm |
| BHQ2 | √ | √ | √dT | 550 nm-650 nm | 579 nm |
| BHQ3 |
| √ |
| 620 nm-730 nm | 672 nm |
Common Fluorophores and Quenchers Pairs
| Description | Taqman Quenchers | MGB Quenchers | Molecular Beacon Quenchers |
| BHQ1 | BHQ2 | BHQ3 | MGB | Dabcyl |
| Pacific Blue |
|
|
|
|
|
| Atto 425 | √ |
|
| √ | √ |
| BODIPY FL | √ |
|
| √ | √ |
| FAM | √ |
|
| √ | √ |
| Alexa Fluor 488 | √ |
|
| √ | √ |
| TET | √ |
|
| √ | √ |
| JOE | √ |
|
| √ | √ |
| Yakima Yellow | √ |
|
| √ | √ |
| VIC | √ |
|
| √ | √ |
| HEX | √ |
|
| √ | √ |
| Quasar 570 |
| √ |
|
|
|
| Cy3 |
| √ |
|
|
|
| NED |
| √ |
|
|
|
| TAMRA |
| √ |
|
|
|
| ROX |
| √ |
|
|
|
| AquaPhluor 593 |
| √ |
|
|
|
| Texas red |
| √ |
|
|
|
| Atto 590 |
| √ |
|
|
|
| Cy5 |
|
| √ |
|
|
| Quasar 670 |
|
| √ |
|
|
| Cy5.5 |
|
| √ |
|
|
【Product List】
| General
primer | Classification | DNA Length |
| Conventional Primer Synthesis | 11-59mer |
| Explaination | All products are purified by HPLC |
| Product Type | Modifications | Product Type | Modifications |
| Single-labeled
Probes | LNA(bp) | Dual-labeled
Probes | 5′ FAM 3′ TAMRA
|
| DI(bp) | 5′ FAM 3′ BHQ1 |
| DU(bp) | 5′ FAM 3′ Dabcy1 |
| sufo(bp) | 5′ FAM 3′ MGB |
| Spacer C3(bp) | 5′ FAM 3′ Eclipse |
| Spacer 9(bp) | 5′ HEX 3′ BHQ1 |
| Spacer 12(bp) | 5′ HEX 3′ TAMRA |
| Spacer 18(bp) | 5′ HEX 3′ Eclipse |
| dSpacer | 5′ HEX 3′ Dabcy1 |
| dT-C6-Amino | 5′ HEX 3′ MGB |
| 5′ -SH | 5′ ROX 3′ BHQ2 |
| 5′ NH2 C6 | 5′ ROX 3′ MGB |
| NH2 C12 | 5′ Cy5 3′ BHQ2 |
| 3′ NH2 C7 | 5′ Cy5 3′ BHQ3 |
| 5′ -Phosphorylation | 5′ Cy5 3′ MGB |
| 3′ -Phosphorylation | 5′ Cy5.5 3′ BHQ3 |
| 5′ Biotin | 5′ TET 3′ BHQ1 |
| 3′ Biotin | 5′ TET 3′ TAMRA |
| 5′ FAM | 5′ VIC 3′ Dabcy1 |
| 3′ FAM | 5′ VIC 3′ BHQ1 |
| 5′ HEX | 5′ VIC 3′ TAMRA |
| 5′ TET | 5′ VIC 3′ MGB |
| 5′ TAMRA | (dT) probes | dT-FAM |
| 3′ TAMRA | dT-ROX |
| 5′ ROX | dT-TAMRA |
| 3′ ROX | dT-Dabcy1 |
| 5′ Cy3 | dT-Cy3 |
| 3′ Cy3 | dT-Cy5 |
| 5′ Cy5 | Single-labeled
Probes | 3′ -Dabcy1 |
| Cy5.5 | 5′ -Dabcy1 |
| 3′ Cy5 | 3′ BHQ1 |
| 5′ VIC | 3′ BHQ2 |
3′ddA/ddT/ddC/ddG | 3′ BHQ3 |
| 5′rapp | 3′ eclipse |
| 3′ -MGB |
*Other modification types are upon requested.