Welcome to DIAGREAT!
Hotline: 86 010-69502385
Products Products
Product name

MTX

Release time 2018-12-08
Clicks 34

Product Name: Methotrexate (Immunochromatographic Assay

Abbreviation: MTX

 

APPLICATION:

For the quantitative determination of Methotrexate in human serum, plasma or whole blood.

Methotrexate is a folic acid antitumor drug that widely used in the treatment of malignant tumors such as acute lymphoblastic leukemia, lymphoma, chorionic epithelial cancer and osteosarcoma. High-dose methotrexate combined with calcium leucovorin is one of the important treatment for acute lymphoblastic leukemia, malignant lymphoma and osteosarcoma. High-dose methotrexate chemotherapy requires MTX blood concentration monitoring to guide the rational use of rescue drugs and avoid the serious side effects caused by MTX. Therefore, the monitoring of methotrexate blood concentration is a great significance for guiding the clinical rational use of drugs in cancer patients.

 

PRCINCIPLE:

Take the sample to the strip, The MTX in the specimen binds to an antibody coated with a fluorescent particle coated on the glass fiber to form a fluorescent particle-antibody-antigen complex. The immune complex is chromatographed along the nitrocellulose membrane to the detection zone (T), which binds to the pre-coated MTX-BSA conjugate and its fluorescence intensity is inversely proportional to the MTX content of the sample. The anti-IgY fluorescent antibody particles are chromatographed to the quality control zone (C) and bound to the pre-coated Organic IgY.

 

Method:

Time-Resolved Immunofluorescence Chromatography

Assay Time: 15min

Sample Volume: 20µl

Sample Type: Serum / Plasma / Whole blood

 

Technical parameters:

Analytical Sensitivity:0.05μmol/L

Measuring Range: 0.05 ~ 20μmol/L

Precision: Intra-assay CV<10%, Inter-assay CV<15%.

Accuracy: Relative deviation of Quality control<10%

Stability: Stable up to 18 months if stored at 2 - 30°C in its sealed pouch.

 

STORAGE AND STABILITY:

The strip is stable for 18months (while sealed in an aluminum foil pouch) if stored at 2 ° C - 30 ° C, the fluorescent antibody in a tube is stable for 18months if stored at 2 ° C - 8 ° C, and the dissociation liquid in a tube is stable for 18months if stored at 2 ° C - 30 ° C. After the strip is opened, the test should be performed in 1 hour.

The date of manufacture is detailed in the label.

 

Package:

25 pieces/box

Name

Composition

Strip

Consisting of a cartridge and a strip, the test strip comprises a sample pad, a glass fiber (Coated with fluorescent particles -MTX antibody conjugate, fluorescent particle - IgY antibody conjugate), nitrocellulose membrane (detection zone coated with MTX-BSA conjugate, quality control zone coated with organic IgY), absorbent paper, PVC board.

Magcard

Have Calibration curve information of this batch reagents.

Buffer

Main component is phosphate buffered saline (PBS).

QC(Optional)

Freeze-dried products, the target value of each batch is shown in the target value list.

Manual

To descripe basic information, usage methods and precautions

 

 

Applicable POCT Analyzer

DG-1

MTX

Recommend / Products MORE+
Links:
  • Your name:
  • *
  • Your phone:
  • *
  • Your mailbox:
  • *
  • Your needs:
  • *
     
Address: three floor, new Hualian science and technology building, 5 jiaxhuang Road, Tongzhou District, Beijing.
Telephone: 86 010-69502385
Fax: 86 010-69507615
Zip code:330520
Copyright ©2018 - 2019  DIAGREAT
Rhino cloud provides enterprise cloud services
犀牛云提供云计算服务