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Product name

HFABP

Release time 2018-12-08
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Product Name: H-Fatty Acid Binding Protein (Immunochromatographic Assay

Abbreviation: HFABP

 

APPLICATION:

For the quantitative determination of cardiac fatty acid binding protein in human serum, plasma or whole blood.

HFABP has a molecular weight of 15 kDa and is a rich novel small cytoplasmic protein in the heart. After the onset of myocardial ischemic injury, HFABP can be found in the blood as early as 1-3 hours after the onset of chest pain, peaking at 6-8 hours and plasma levels returning to normal within 24-30 hours. HFABP is a great significance in the diagnosis of acute myocardial infarction, myocardial injury and acute coronary syndrome (ACS), especially in the diagnosis of ACS with troponin.

 

PRCINCIPLE:

Take the sample to the strip, The HFABP in the specimen binds to the antibody coated with the fluorescent particles coated on the glass fiber to form a fluorescent particle-antibody-antigen complex. The immune complex is further chromatographed along the nitrocellulose membrane to the detection zone (T), and combined with the pre-coated mouse anti-CK-MB monoclonal antibody, the fluorescence intensity is proportional to the CK-MB content in the sample, and the remaining Fluorescent antibody particles were chromatographed into the control region (C) and bound to pre-coated goat anti-mouse IgG.

 

Method:

Time-Resolved Immunofluorescence Chromatography

Assay Time: 15 min

Sample Volume: 20μL

Sample Type: Serum / Plasma / Whole blood

 

Technical parameters:

Reference Range: 0-7ng/ml

Analytical Sensitivity: 1ng/ml

Measuring Range: 1 ~ 100 ng/mL

Precision: CV≤15%

Stability: Stable up to 18 months if stored at 2 - 30°C in its sealed pouch.

 

STORAGE AND STABILITY:

The strip is stable for 18months (while sealed in an aluminum foil pouch) if stored at 2 ° C - 30 ° C, the fluorescent antibody in a tube is stable for 18months if stored at 2 ° C - 8 ° C, and the dissociation liquid in a tube is stable for 18months if stored at 2 ° C - 30 ° C. After the strip is opened, the test should be performed in 1 hour.

The date of manufacture is detailed in the label.

 

Package:

25 pieces/box

Name

Composition

Strip

Consisting of a cartridge and a strip, the test strip comprises a sample pad, a glass fiber (coated with a fluorescent particle- HFABP antibody conjugate), a nitrocellulose membrane (the detection zone is coated with a HFABP monoclonal antibody, and the quality control zone is coated with a sheep) Anti-mouse IgG), absorbent paper, PVC board.

Magcard

Have Calibration curve information of this batch reagents.

Buffer

Main component is phosphate buffered saline (PBS).

QC(Optional)

Freeze-dried products, the target value of each batch is shown in the target value list.

Manual

To descripe basic information, usage methods and precautions

 

 

Applicable POCT Analyzer

DG-1

HFABP

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